The negative holder that comes with the unit does not have spaces for 110 negatives. Epson doesn't make one, and I don't know if there is an aftermarket product you can use. There is a workaround but I don't know how well it works. I got it from the Epson website: Yes. However, a 110 film holder is not available to scan this film size (16mm). To scan 110 film, place it face-down on the document table. (Your images and any wording on the film strips should appear backwards on the side that faces up). Be sure to position the film directly underneath the transparency unit window located in the scanner cover.