That is actually the reason I bought it, updating my old V100 which was 35mm only. My wife found some old 120 film negatives of her as a child she had never seen. Initial quote from camera store at $0.25 each to print was reasonable until I brought them in and discovered was not chemical based processing but needed to be digitized at $5 each to inkjet process. Did the math and that paid for a new scanner! It is not really a tray but the V550 comes with two plastic guide holders - one 35mm the other 120mm film/slides - that lays on the glass platten with the side tabs positioning it correctly. 600,616,and 620 are the 2" wide 120 film. Just a matter if 2 or 3 can go on the platic guide.The white pad in the lid pulls out and exposes the lighting unit for negative/slides which the film in the plastic guide will be directly under. Within the software, I use the "Professional Mode". When you select film as the document type, the resulting scan is a positive, so no other software is required...but I do use Photoshop later for cleanup and cropping before printing. I upped the scan to 1200 dpi for both slides and negatives to give large enough file to enlarge from such a small scan area. Also, while suppose to "smartly" identify breaks for one scan, but had problems recognizing the breaks. Loaded 3 negatives, previewed, selected and scanned individually